To show the organization of the course that includes this module, follow this link Course organization
The course aims to give the students notions of the use of plants for the production of high-value molecules. In particular, the training course will be focused on the techniques used to express recombinant proteins in plant systems with highlights on qualitative and quantitative optimization of recombinant protein expression.
Specific representative cases of molecules produced in plant systems and their applications in the medical and diagnostic fields will be shown and investigated.
1 An introduction to plant molecular farming, state of the art, applications and perspectives.
2 Transient and stable expression systems for recombinant protein production in plant systems.
3 Nanobiotechnology for the production of nano-materials in plant systems and their applications.
4 Plants in molecular farming: bioreactors or direct oral delivery systems.
5 Strategies for the optimization of heterologous proteins in plant systems: from gene construct design to sub-cellular targeting.
6 Downstream processing in plant molecular farming
7 Glycosilation and glycol-engineering in plant systems for the production of heterologous proteins carrying “humanized” post-translational modifications.
8 In planta production of complex molecules: the antibodies.
9 Examples of recombinant protein expressed in plant systems (Vaccines and antibodies).
The final exam consists in an oral test on the topics of the course and on a written report on the scientific literature dealing with the production of a specific molecule in plant systems.
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