Omics sciences - MODULO I (2017/2018)



Course code
4S003719
Credits
6
Academic sector
AGR/07 - AGRICULTURAL GENETICS
Language of instruction
Italian
Teaching is organised as follows:
Activity Credits Period Academic staff Timetable
Metabolomica teoria 2 II sem. Flavia Guzzo

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Trascrittomica laboratorio 3 II sem. Diana Bellin

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Trascrittomica teoria 1 II sem. Diana Bellin

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Learning outcomes

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MM: Metabolomica teoria
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The main objective of the course is to introduce the metabolomics analysis as an instrument to investigate the biological complexity of cells/tissues/organs/organisms from the point of view of the small molecules, that are the intermediate and end products of the metabolism. The metabolomics approach will be described as pipeline, a chain of connected steps from the experiment design up to the analysis and the data modeling.
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MM: Trascrittomica laboratorio
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MM: Trascrittomica teoria
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Syllabus

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MM: Metabolomica teoria
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-the experimental design; -metabolite extraction and preparation for LC-MS; -data formats and conversion; -chromatogram analysis and metabolite annotation; -data preprocessing; the effect of the technique of data normalization; -data processing (with MZmine): m/z feature extraction, chromatogram deconvolution and alignement; production of the Feature Quantification Matrix; -data analysis through multivariate analysis (PCA, PLS-DA, OPLS-DA) and univariate analysis.
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MM: Trascrittomica laboratorio
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MM: Trascrittomica teoria
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Assessment methods and criteria

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MM: Metabolomica teoria
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The examination will be an oral test aimed to verify : -the overall degree of knowledge of the subject of this course; -the scientific language skills.
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MM: Trascrittomica laboratorio
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MM: Trascrittomica teoria
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STUDENT MODULE EVALUATION - 2017/2018