Plant biology and physiology (2017/2018)

Course code
4S02734
Credits
12
Coordinator
Tiziana Pandolfini
Teaching is organised as follows:
Unit Credits Academic sector Period Academic staff
BIOLOGIA VEGETALE 6 BIO/01-GENERAL BOTANY I sem. Flavia Guzzo
FISIOLOGIA VEGETALE 6 BIO/04-PLANT PHYSIOLOGY II sem. Tiziana Pandolfini

Learning outcomes

------------------------
MM: BIOLOGIA VEGETALE
------------------------
This course will provide students with an overview of plant structure and organization, at four organization levels, corresponding to cells, tissues, organs, and whole plants in their environments.
------------------------
MM: FISIOLOGIA VEGETALE
------------------------
The course examines the principal physiological and molecular aspects of plant growth and development. The objective of the course is the acquisition of the theoretical fundamentals of plant physiology relevant for viticulture. After attending the course, the students will be able to comprehend the following physiological processes: water balance, xylematic and floematic transport, carbon metabolism, the role of phytohormones in plant growth and development. Laboratory To acquire knowledge about some experimental techniques used in plant physiology and to carry out a laboratory experience using plant material. The students will learn how to set up a laboratory in vitro test using plant tissues and how to collect, analyze and present the data.

Syllabus

------------------------
MM: BIOLOGIA VEGETALE
------------------------
1)The plant cell: the membrane system; the cytosol and the ribosomes; the cell wall; cytoskeleton; endomembrane and secretory pathway; the vacuole and the cell expansion process; plastids and mitochondria; the nucleus, mitosis and meiosis, the cytokinesis. 2) The plant tissues primary and secondary meristems; parenchymatic tissues; tegument tissue; conducting and supporting tissues; secretory tissues. 3) The plant anatomy and overall organization The shoot and its development; the stem, primary and secondary organization; the leaves and their adaptation to the environment; the root and its development; flowers, fruits and seeds; reproductive botany.
------------------------
MM: FISIOLOGIA VEGETALE
------------------------
Water transport in plants: chemical properties of water, water and solute transport processes. Water balance of plants: movement of water in the soil, water absorption by roots, water transport through the xylem, transpiration and stomatal movements, control of stomatal opening. Water stress: physiological responses to drought. Photosynthesis: general concepts of light reactions and carbon reactions. Starch and sucrose synthesis. Translocation in the phloem: phloem loading and unloading, the sink-source concept, allocation and partitioning of phosynthates. General concepts of genetic information, gene expression and signal transduction pathways in eukaryotes. Photomorphogenesis: phytochrome, phytochrome responses, cellular and molecular mechanisms. Blu-light responses. Phytohormones: biosynthesis, physiological effects and molecular modes of action of auxin, cytokinins, gibberellins, ethylene, abscisic acid , brassinosteroids, polyamines, jasmonic acid. Laboratory To acquire knowledge about some experimental techniques used in plant physiology. Use of reporter genes for gene expression analysis with particular attention to the study of hormone distribution in response to different stimuli; Agrobacterium tumefaciens as natural genetic engineer: generation of transgenic plants by Agrobacterium and biolistic methods. Introduction to in vitro culture of plant tissues. Practical work 1) Measurement of water potential in plant tissues. 2)The effects of auxin on adventitious root formation.

Assessment methods and criteria

------------------------
MM: BIOLOGIA VEGETALE
------------------------
The examination will be an oral test aimed to verify : -the ability to recognize plant anatomical and histological structures; this test will be performed through the description of an anatomical/histological structure, included in the Plant Anatomical Atlas (author: Flavia Guzzo), which is supplied free of charge to the students through the e.learning platform of UNIVR; -the overall degree of knowledge of the subject of this course; -the scientific language skills.
------------------------
MM: FISIOLOGIA VEGETALE
------------------------
The exam will ascertain: Students’ knowledge on the topics of the lectures; The language accuracy used to expose the arguments; The ability to link the knowledge acquired to what learned from the Plant Biology course. For both attending and non-attending students, the oral examination will concern the topics discussed in the lessons and covered in the text book. Laboratory A written report describing the results of the laboratory experiences.

Reference books
Author Title Publisher Year ISBN Note
Taiz L. Zeiger E. Elementi di Fisiologia Vegetale Piccin 2013
N. Rascio Elementi di Fisiologia Vegetale EdiSES 2012 9788879597104
J D Mauseth Botanica Fondamenti di biologfia delle piante Idelson-Gnocchi 2014 9788879475822
Gabriella Pasqua e altri Botanica generale e diversità vegetale Piccin 2011 9788829920600