Molecular analytical methods - GENOMICA E TRASCRITTOMICA (2010/2011)

Course code
4S02774
Credits
3
Other available courses
Other available courses
    Academic sector
    CHIM/01 - ANALYTICAL CHEMISTRY
    Language of instruction
    Italian
    Teaching is organised as follows:
    Activity Credits Period Academic staff Timetable
    Teoria 1 I semestre Mario Pezzotti
    Laboratorio 2 I semestre Mario Pezzotti

    Lesson timetable

    I semestre
    Activity Day Time Type Place Note
    Teoria Tuesday 9:30 AM - 11:30 AM lesson Lecture Hall H from Nov 30, 2010  to Jan 31, 2011
    Laboratorio Friday 1:30 PM - 2:30 PM lesson Laboratory Laboratorio di Biotecnologie genetiche from Nov 30, 2010  to Jan 31, 2011
    Laboratorio Friday 2:30 PM - 7:30 PM lesson Laboratory Laboratorio di Biotecnologie genetiche from Nov 30, 2010  to Jan 31, 2011

    Learning outcomes

    Genomics and transcriptomics can be considered as the new frontier of modern genetics research, they are characterized by a huge complexity derived by the use of dedicated equipments able to produce an enormous data in a short time experiment. From medicine to experimental biology passing through agriculture the use of genomic tools is capillary diffuse for the modern interpretation of biological processes.
    The aim of this course is to illustrate in depth the technological aspects of genomics and transcriptomics by comparing different update methodologies and to perform an real experiment of global analysis of gene expression by using to different microarray platform.

    Syllabus

    Theory

    Genomics and its tools
    Transcriptomics and its tools
    Comparison of transcriptomic platforms
    Pros and contra of different transcriptomic platforms


    Laboratory

    RNA extraction from plant tissue
    RNA quality control by bioanalyzer
    RNA labelling with two different methods (Combimatrix e NimbleGen).
    Ibridization of two different array chips (Combimatix e NimbleGen)
    Microarray scanning by confocal laser scanner
    Data extraction and analysis

    Assessment methods and criteria

    Oral examination