|Teoria||1||I semestre||Mario Pezzotti|
|Laboratorio||2||I semestre||Mario Pezzotti|
|Teoria||Tuesday||9:30 AM - 11:30 AM||lesson||Lecture Hall H||from Nov 30, 2010 to Jan 31, 2011|
|Laboratorio||Friday||1:30 PM - 2:30 PM||lesson||Laboratory Laboratorio di Biotecnologie genetiche||from Nov 30, 2010 to Jan 31, 2011|
|Laboratorio||Friday||2:30 PM - 7:30 PM||lesson||Laboratory Laboratorio di Biotecnologie genetiche||from Nov 30, 2010 to Jan 31, 2011|
Genomics and transcriptomics can be considered as the new frontier of modern genetics research, they are characterized by a huge complexity derived by the use of dedicated equipments able to produce an enormous data in a short time experiment. From medicine to experimental biology passing through agriculture the use of genomic tools is capillary diffuse for the modern interpretation of biological processes.
The aim of this course is to illustrate in depth the technological aspects of genomics and transcriptomics by comparing different update methodologies and to perform an real experiment of global analysis of gene expression by using to different microarray platform.
Genomics and its tools
Transcriptomics and its tools
Comparison of transcriptomic platforms
Pros and contra of different transcriptomic platforms
RNA extraction from plant tissue
RNA quality control by bioanalyzer
RNA labelling with two different methods (Combimatrix e NimbleGen).
Ibridization of two different array chips (Combimatix e NimbleGen)
Microarray scanning by confocal laser scanner
Data extraction and analysis