Biomolecular Technology - Modulo 2 - Laboratorio (2008/2009)

Course partially running (all years except the first)

Course code
4S00175
Name of lecturer
Barbara Molesini
Number of ECTS credits allocated
3
Academic sector
BIO/11 - MOLECULAR BIOLOGY
Language of instruction
Italian
Period
1° Sem dal Oct 1, 2008 al Feb 6, 2009.

To show the organization of the course that includes this module, follow this link * Course organization

Lesson timetable

Learning outcomes

Southern-blot (based on hybridization between nucleic acids) and RT-PCR (based on PCR) performed on transgenic and wild-type plants, will allow the student to apply the knowledges acquired.

Syllabus

1) SOUTHERN BLOT ANALYSIS OF TRANSGENIC PLANTS: GENOMIC DNA EXTRACTION, QUANTIFICATION, RESTRICTION DIGESTION, ELECTROPHORESIS OD DIGESTED DNA, CAPILLARY BLOTTING, FIXING THE DNA TO THE MEBRANE, LABELING NONRADIOACTIVE PROBE, PREHYBRIDIZATION, HYBRIDIZATION, WASHING, DETECTION OF HYBRIDS BY CHEMIOLUMINESCENT SUBSTRATE

2) RT-PCR: RNA EXTRACTION, QUANTIFICATION, DNase TREATMENT, SYNTHESIS OF cDNA, PCR

Assessment methods and criteria

Delivery of a detailed report regarding the experiments performed in the laboratory.

Studying