Biomolecular Technology - Laboratorio (modulo 2) (2007/2008)

Course partially running (all years except the first)

Course code
4S00175
Name of lecturer
Barbara Molesini
Number of ECTS credits allocated
3
Academic sector
BIO/11 - MOLECULAR BIOLOGY
Language of instruction
Italian
Period
1° Sem dal Oct 1, 2007 al Jan 23, 2008.

To show the organization of the course that includes this module, follow this link * Course organization

Lesson timetable

Syllabus

1) SOUTHERN BLOT ANALISIS OF TRANSGENIC PLANTS: GENOMIC DNA EXTRACTION, QUANTIFICATION, RESTRICTION DIGESTION, ELECTROPHORESIS OD DIGESTED DNA, CAPILLARY BLOTTING, FIXING THE DNA TO THE MEBRANE, LABELING NONRADIOACTIVE PROBE, PREHYBRIDIZATION, HYBRIDIZATION, WASHING, DETECTION OF HYBRIDS BY CHEMIOLUMINESCENT SUBSTRATE

2) RT-PCR: RNA EXTRACTION, QUANTIFICATION, DNase TREATMENT, SYNTHESIS OF cDNA, PCR

Studying